For students

Camp Pathological

A summer program for high school students offering hands-on activities about clinical research.

Article Groups

Travis Group:

Hector
Linda
Lawrence
Issak
Heather
Denesha
Cindy

Liz Group:

Toi-Lisa
Jaron
Malissa
Domonique
Jonyae
Zakiyat

Zulma Group: (zperez@kumc.edu)

Letitia
Stephanie
Quran
Pazong
Victor
Jessica

Keith Group:

Dorthy
Brytani
Matthew
Susann
Olivia
Jennifer

Bromothymol Blue and Respiration

Summary: 
Students will compare how much carbon dioxide they breathe out before exercise and after. They will then create mini investigations to determine if other factors may influence the amount of CO2 produced by comparing person with asthma to a non-asthmatic, couch potato to an athlete. Students will breathe into a flask with the indicator, bromothymol blue (BTB), and then titrate it to the original color with weak sodium hydroxide (NaOH).
Learning Objectives: 
Compare the amount of carbon dioxide in a person’s breath before and after exercise.
Investigate other factors that may influence how much carbon dioxide is exchanged in the lungs.
Graph and analyze the data.
Draw conclusions about factors that influence how much carbon dioxide is exchanged in the respiratory system based on data and observations.
Identify, plan and conduct other investigations that relate to this inquiry.
Relate the structures and functions of the respiratory system that are involved in the exchange of gases.
Appropriate for: 
10th
11th
12th
Class Time: 
one hour
Materials and Supplies: 

  • Stock Bromothymol Blue  (BTB)  (Dilute .04% Bromothymol Blue Indicator Solution to .004%.  When the flask is sitting on printed materials, you should be able to easily read the words through the pale blue solution.  If the stock is greenish instead of blue,  add weak NaOH to shift the solution to be more blue.)  Each test group will need about 450 to 500 mls of diluted solution.

Per test group:  

  • 2 Erlenmeyer flasks (250 ml)
  • 1 small beaker (100 ml)
  • 3 to 5 straws per student who tests
  • Weak sodium hydroxide in a dropper bottle  (.1 M NaOH)
  • Graduated cylinder (100 ml)
  • Timer or clock with a second hand
  • Safety eye wear
Background Information and Resources: 

Students should not ingest the BTB and safety eye wear is recommended. See:  MSDS  http://www.flinnsci.com/Documents/MSDS/B/BrthyBIS.pdf

See student handout:  BROMOTHMOL BLUE (BTB) and RESPIRATION for detailed description of the activity.

See:  Lab Activity Checklist to evaluate how the teams collaborated and drew conclusions about the factors that influence carbon dioxide exchange in the respiratory system.

The color change is an indication of the amount of acid or base there is in the solution.  The more dissolved carbon dioxide from the participant’s lungs, the more acidic it is.  The faster it changes colors the more acidic it is.  Carbon dioxide dissolves in the water to make carbonic acid (H20 + CO2 > H2CO3).  The pH range for BTB is approximately 6.2 to 7.6.  It is yellow when acidic and blue when basic.  To see a color chart go to: 

www.mysciencebox.org/files/bromthymol_blue.doc

The student will develop the abilities necessary to do scientific inquiry and develop an understanding of scientific inquiry.

Identify questions and concepts that guide scientific investigations

Design and conduct scientific investigations.

Use technology and mathematics to improve investigations and communications

Formulate and revise scientific explanations and models using logic and evidence

Communicate and defend a scientific argument

Use mathematics in all aspects of scientific inquiry.

The student will develop an understanding of the cell, molecular basis of heredity, biological evolution, interdependence of organisms, matter, energy, and organization in living systems, and the behavior of organisms.

 Most cell functions involve chemical reactions

Cells can differentiate, and complex multicellular organisms are formed as a highly organized arrangement of differentiated cells.

Organisms have behavioral responses to internal changes and to external stimuli.  

The student will develop an understanding of personal and community health.

Making a stock solution:

  1. Most purchased solutions are 0.04% solutions.  Dilute this solution so that the participants can read text through the bottom of the flask when it contains the diluted solution.  (See materials section above.)  Prepare enough stock to have each team to use about 500 mls of solution.
  2. Students may need extra straws after exercising.  Ask students to practice breathing in through their mouth and noses but only exhaling through the straws inserted in the solution.  Caution them not to inhale or draw the solution into their mouths.  

Outbreak Simulation

Summary: 
Teams of students participate in a 2 round simulation that begins with an outbreak of smallpox in Kansas City. Each student assumes a role –a news reporter, a doctor at a local hospital, a grocery store owner (civilian), an expert in disease, the governor and the President of the United States. Each faces a dilemma which does not have a clear, obvious answer. The students discuss and come to the best decision they can only to find another dilemma facing them again. The students again are challenged to make the best decision. All debrief about the process and the situational ethics evident in the simulation.
Learning Objectives: 
Identify factors that may help prevent, control or cure the disease in the scenario.
Participate in a role play scenario that involves making decisions for others and exploring the steps and knowledge needed to make an ethical decision.
Recognize that some decisions can not be made only on a “medical” level but has pros and cons that will affect the patient and family in other ways.
Identify pros and cons of the hazards present and the danger and risk involved in each decision.
Identify ways to resolve the types of conflicts faced before the next time similar situations come up (suggest clinical trials or activities).
Appropriate for: 
10th
11th
12th
Class Time: 
2 class periods
Materials and Supplies: 

Student handout: 1 copy of roles and dilemmas from Outbreak Simulation handouts Facilitator’s handouts (Brief #1 and Brief #2) found in Outbreak Simulation handout

Large paper to chart decisions and evidence for each decision

Markers

Background Information and Resources: 

Background information sheet:  The Real Outbreak exercise:  “Operation Dark Winter” includes link to materials about this exercise held in 2001.

 

Resources

Outbreak Simulation adapted from: http://www.upmc-biosecurity.org/website/events/2001_darkwinter/

More information about ethical decisions in biomedical fields: http://www.brown.edu/Departments/Center_for_Biomedical_Ethics/overview.html

Background information about smallpox: http://www.bt.cdc.gov/agent/smallpox/disease/

Clinical symptoms of smallpox: http://www.bt.cdc.gov/agent/smallpox/overview/disease-facts.asp

Information regarding the smallpox vaccine: http://www.bt.cdc.gov/agent/smallpox/disease/

Information about the military smallpox vaccine program: http://www.smallpox.mil/

Teaching notes:

There are a few key points that should be evident through this activity:

  • In the United States, a significant portion of the population does not have adequate access to healthcare. This would make a situation like a bioterrorist attack even worse, as the country's healthcare infrastructure is not capable of taking care of its citizens during peacetime and under more stable conditions. 
  • The United States does not have an effective supply of vaccines or medication to deal with a variety of scenarios if there were an Toutbreak of disease.  It also takes a significant amount of time to make new vaccines using present methods, which would prolong the time in which the disease could spread
  • Vaccines have changed the entire world through reducing the incidence and thus prevalence of many debilitating diseases.  In the case of smallpox, vaccination campaigns were even able to eradicate the virus from the planet, except for within laboratories and tightly-controlled scientific settings.  This also means that healthcare personnel are not trained to deal with diseases that have not been seen in their country for almost their entire lifetime.
  • Military interests are very closely tied to domestic interests, even if they do not always seem to be so at face value.

Setting up the scenario:  

1.  Cut apart roles and dilemma #1 and #2. Keep them in 2 groups. Place chart paper on the wall for recording the decisions.

2.  Divide the class into groups of 6 and select roles for the scenario. (A group of 7 if one will be the person who charts the decisions and acts as facilitator.)

3.  Each will read their own Roles and background position about how their policies or convictions would influence their decision making.  After absorbing this information individually,  everyone will be briefed on the developing medical situation.  The facilitator or teacher will read BRIEF #1 to the group.

4.  The facilitator will pass out Dilemma #1 for each role.  The dilemma is unique to their role and individually the student must make what s/he think is the best decision. Once each student has had sufficient time to read, consider and decide, s/he will write down the decision  and a brief rational so that it can be held up and shared with the group later in the role play.

5.  All members of the group will then briefly describe to each other who they are, their dilemma, which decision they made and why. The facilitator will record the decisions on the chart paper.  They will then discuss together the implications of each person’s decision with regards to the other players, and the U.S. population. 

6.  The facilitator will read  BRIEF #2.   There will then be a second brief, The facilitator will pass out Dilemma #2 for each role.  and each role will be presented with a similar but more complicated or difficult dilemma.  The same process of reporting and discussing implications will conclude the exercise.

Debriefing – whole class:

7.  Bring the different teams together and compare and contrast the decisions made,  the evidence used and the possible 2nd and 3rd order consequences of the decisions on the local, regional, national and international communities.

8.  View the Briefing slides (http://www.upmc-biosecurity.org/website/events/2001_darkwinter/dark_winter_slides.html) from Operational Dark Winter and the concerns and strategies used in the mock disaster.  See also http://www.upmc-biosecurity.org/website/events/2001_darkwinter/   for the overview,  summary, and script of the original simulation developed for the national government.

9.  Complete student self evaluation and group evaluation using rubric.

10.  Extend the simulation by completing the Table Top Emergency Response – Anthrax Release

Respiratory Organ Location: Draw a Breath

Summary: 
Students will fill in as many organs and what they do on a line drawing of the upper torso. After studying about the structure and function of the respiratory system, they will draw in the organs and what they do on an identical line drawing. The 2nd drawing will be scored with a rubric. (suggested use: formative and summative assessment)
Learning Objectives: 
Draw the organs involved in respiration on a line drawing at approximately the correct location and size.
Label the organs correctly.
Provide correct information about the structure and function of the organs and their processes.
Appropriate for: 
10th
11th
12th
Class Time: 
Total of two class times (separated by teaching and learning about the respiratory system)
Materials and Supplies: 
  • Poster paper or large sheet of brown paper with the head and torso outline of a human body drawn in the middle. One per student or one per team.
  • Markers
  • Resource diagrams and materials once the first drawing is completed
  • Handout(s): grading rubric (created by the teacher) and other handouts as needed (answers/diagrams/pictures)
Background Information and Resources: 
  • Project it on to a poster page or sheet of brown paper.  Trace the outline of the head, upper arms and torso.  Do this for each team or each student.  You will need a second tracing of the same drawing for the summative assessment. (suggestion:  Use a different color to outline the first and the second to easily tell the pre and post drawings.)
  • Rubric handout for assessing the second drawing.
  • Allow time to trace the outlines needed for the size of the class.
  • Consider taking digital pictures or saving work to scan in and post online.

Discover what students do and don’t know about the respiratory system. 

  1. Give each pair or student an outline of a human torso and several markers.  
  2. Ask them to draw the organs and structures that carry the air from outside the body to where it is absorbed into the blood stream.  
  3. Once they have drawn as many structures as they can, they should name them and describe what they know about the process and/or structure.     
  4. Present and review the pathway of the air in and out during the next several lessons correcting any misconceptions.
  5. Repeat the process as a check for understanding by repeating the process and grading with the rubric.     
Assessment: 
Score second drawing with a rubric created by the teacher.
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